Figure 1.

Purification of the FANCD2 Protein Complex Containing WRNIP1

(A) The FANCD2 complex was purified from HeLa cells. Proteins were resolved via SDS–PAGE and visualized using silver stain.

(B) Three groups of proteins identified in the purified complexes. DDR refers to DDR proteins not primarily associated with ICL repair. See Table S1 for full list of proteins.

(C) Positions of peptides (shown in red) originating from WRNIP1 following MS/MS analysis of purification in (A).

(D) Coomassie blue staining of the recombinant proteins used in the in vitro protein-protein interaction assay shown in (E).

(E) Immunoblot analysis of the in vitro protein-protein interaction assay showing direct interaction between WRNIP1 and FANCD2.