REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-FANCD2 (FY17)	Santa Cruz Biotechnology	Cat# sc-20022; RRID: AB_2278211
Mouse monoclonal anti-α-Tubulin (DM1A)	Merck-Millipore	Cat# 05-829; RRID: AB_310035
Mouse monoclonal anti-WRNIP1 (WHIP A8)	Santa Cruz Biotechnology	Cat# Sc-376438; RRID: AB_11149006
Mouse monoclonal anti-FLAG (M5)	Sigma-Aldrich	Cat# F4042; RRID: AB_439686
Mouse monoclonal anti-HA	Roche	Cat# 11583816001; RRID: AB_514505
Mouse monoclonal anti-Ubiquitin (FK2)	Merck-Millipore	Cat# ST1200; RRID: AB_2043482
Mouse monoclonal anti-poly-Histidine	Sigma-Aldrich	Cat# H1029; RRID: AB_260015
Anti-IL-2 Rα Antibody, clone 7G7/B6	Merck-Millipore	Cat# 05-170; RRID: AB_309642
Anti-Mouse (Horseradish Peroxidase conjugated)	GE Healthcare	NA9310V
Bacterial and Virus Strains
Top10	Thermo Fisher	Cat# 404010
Chemicals, Peptides, and Recombinant Proteins
FuGENE6	Promega	Cat# E2691
FLAG-HA-FANCD2	Liang et al., 2016	N/A
His-Strep(II)-WRNIP1	This paper	N/A
His-MBP	Motnenko et al., 2018	N/A
Protein A Sepharose Cl-4B	Thermo Fisher	Cat# GE17-0963-02
Dynabeads Goat Anti-Mouse IgG	Thermo Fisher	Cat# 11033
Anti-FLAG M2 agarose resin	Sigma-Aldrich	Cat# A2220
Ni2–NTA agarose resin	QIAGEN	Cat# 30210
Trioxsalen (TMP)	Sigma-Aldrich	Cat# 6137
Mitomycin C from Streptomyces caespitosus	Sigma-Aldrich	Cat# M4287
Benzonase	Sigma-Aldrich	Cat# E1014
Critical Commercial Assays
Sequencing	Source BioScience	N/A
Deposited Data
Mass spectrometry	This paper, and Mendeley Data	Table S1; https://doi.org/10.17632/z7nx93j39j.1
Experimental Models: Cell Lines
HeLa FANCD2−/−	Liang et al., 2016	N/A
HeLa Flp-in T-Rex cells	Thermo fisher	N/A
HeLa Flp-in WRNIP1 −/−	This paper	N/A
HeLa Flp-in WRNIP1-EGFP + mCherry-FANCD2	This paper	N/A
HeLa Flp-in WRNIP1 −/− + WRNIP1-EGFP	This paper	N/A
HeLa FANCD2 −/− WRNIP1−/−	This paper	N/A
HeLa expressing mCherry-UHRF1 and EGFP-FANCD2	This paper	N/A
HeLa expressing mCherry-UHRF1 and EGFP-FANCI	This paper	N/A
HeLa WRNIP1 −/− expressing WRNIP1-EGFP and mCherry-UHRF1	This paper	N/A
HeLa WRNIP1 −/− expressing WRNIP1-19R-EGFP and mCherry-UHRF1	This paper	N/A
HeLa expressing Flag-HA-tagged FANCD2	Liang et al., 2016	N/A
HeLa S3 expressing Flag-HA-WRNIP1	This paper	N/A
HeLa Flp-in WRNIP1 −/− +WRNIP1-N33A-EGFP	This paper	N/A
HeLa Flp-in WRNIP1 −/− +WRNIP1-D37A-EGFP	This paper	N/A
HeLa Flp-in WRNIP1 −/− +WRNIP1-K301R-EGFP	This paper	N/A
HeLa Flp-in WRNIP1 −/− +WRNIP1-K310R-EGFP	This paper	N/A
HeLa Flp-in WRNIP1 −/− +WRNIP1-K335R-EGFP	This paper	N/A
HeLa Flp-in WRNIP1 −/− +WRNIP1-K633R-EGFP	This paper	N/A
HeLa Flp-in WRNIP1 −/− +WRNIP1-K636R-EGFP	This paper	N/A
Oligonucleotides
Primer used to create the Flp-in T-REx HeLa WRNIP1-EGFP cell line (forward primer): 5′-AGTCGGATCCACCATGGAGGTGAGCGGGCCGGAAG-3′	This paper	N/A
Primer used to create the Flp-in T-REx HeLa WRNIP1-EGFP cell line (reverse primer): 5′-AGTCGTCGACGCACCTCCTCTGCTTGAAGAAATCTACC-3′	This paper	N/A
Primer used to create plasmid disrupting the WRNIP1 gene by CRISPR (forward primer): 5′-CACCGGCGAGTTGATGTGCGCGGC-3′	This paper	N/A
Primer used to create plasmid disrupting the WRNIP1 gene by CRISPR (reverse primer): 5′-AAACGCCGCGCACATCAACTCGCC-3′	This paper	N/A
Recombinant DNA
pOZ-N	Nakatani and Ogryzko, 2003	N/A
pBlueScript II SK (+)	Addgene	Cat# 10359-016
pcDNA5-FRT/TO-EGFP	Addgene	Cat# 212205
pOZ-Puro-mCherry	Liang et al., 2016	N/A
pFB-FLAG-HA	Liang et al., 2016	N/A
pFastBac1	Thermo-Fisher	Cat# 10360014
pSpCas9(BB)-2A-Puro (PX459)	Addgene	Cat# 48139
pOG44	Thermo-Fisher	Cat# V600520
Software and Algorithms
Volocity	Quorum Technologies	N/A
Fiji	Fiji.sc	N/A
PyMOL	Pymol.org	N/A
SEQUEST algorithm	Eng et al., 1994	N/A
Other
MS/MS analysis of purified FANCD2 complex (hybrid linear ion trap/FT-ICR mass spectrometer LTQ-FT, Thermo Electron)	Harvard Medical School, Boston	N/A
MS/MS analysis of purified ubiquitinated WRNIP1	Dunn School of Pathology Proteomics Facility	N/A
PE Ultraview Spinning Disk Microscope	Micron Facility, University of Oxford	N/A
Spectrolinker XL-1500 (365 nm)	Department of Biochemistry, University of Oxford	N/A