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. 2020 Jul 10;15(7):e0235856. doi: 10.1371/journal.pone.0235856

Table 1. Primers used for relative quantification of the target gene (mtDNA) and endogenous control (ACTB).

Target gene (Genebank access) Primer Sequence (5’-3’) Product
ACTB (NM_173979.3) ACTB-f GGCACCCAGCACAATGAAGA 67bp
ACTB-r GCCAATCCACACGGAGTACTT
Mt-RNR 2 (AY526085 / AY126697) bMT3010-f GCCCTAGAACAGGGCTTAGT 87bp
bMT3096-r GGAGAGGATTTGAATCTCTGG