Table 1. Primers used for relative quantification of the target gene (mtDNA) and endogenous control (ACTB).
Target gene (Genebank access) | Primer | Sequence (5’-3’) | Product |
---|---|---|---|
ACTB (NM_173979.3) | ACTB-f | GGCACCCAGCACAATGAAGA | 67bp |
ACTB-r | GCCAATCCACACGGAGTACTT | ||
Mt-RNR 2 (AY526085 / AY126697) | bMT3010-f | GCCCTAGAACAGGGCTTAGT | 87bp |
bMT3096-r | GGAGAGGATTTGAATCTCTGG |