Table 1. A list of primers used in this study.

Primer no.	Target gene primer	Primer Sequence	Annealing Temperature Used for PCR	Reference
1	ompW forward	CACCAAGAAGGTGACTTTATTGTG	59 °C	[13]
	ompW reverse	GAACTTATAACCACCCGCG	59 °C	[13]
2	O1-rfb forward	GTTTCACTGAACAGATGGG	54 °C	[15]
	O1-rfb reverse	GGTCATCTGTAAGTACAAC	54 °C	[15]
3	ctxB forward	GGTTGCTTCTCATCATCGAACCAC	60 °C	[16]
	ctxB reverse	GATACACATAATAGAATTAAGGAT	60 °C	[16]
4	zot forward	GCTATCGATATGCTGTCTCCTCAA	62 °C	[17]
	zot reverse	AAAGCCGACCAATACAAAAACCAA	62 °C	[17]
5	toxR forward	ATGTTCGGATTAGGACAC	54 °C	[18]
	toxR reverse	TACTCACACACTTTGATGGC	54 °C	[18]
6	ace forward	AGAGCGCTGCATTTATCCTTATTG	64 °C	[19]
	ace reverse	AACTCGGTCTCGGCCTCTCGTATC	64 °C	[19]
7	rtxA forward	CTG AAT ATG AGT GGG TGA CTT ACG	64 °C	[20]
	rtxA reverse	GTG TAT TGT TCG ATA TCC GCT ACG	64 °C	[20]
8	rtxC forward	CGA CGA AGA TCA TTG ACG AC	58 °C	[16]
	rtxC reverse	CAT CGT CGT TAT GTG GTT GC	58 °C	[16]
9	tagA forward	GGTGGTAAGATATTCACTC	57 °C	[20]
	tagA reverse	GAGACATCTATAGAATACTGGCTG	57 °C	[20]
10	tcpA forward	CACGATAAGAAAACCGGTCAAGAG	64 °C	[14]
	tcpA reverse	ACCAAATGCACGCCGAATGGAGC	64 °C	[14]
11	tcpP forward	ACTCTGTGAATATCATCCTGCC	59 °C	[21]
	tcpP reverse	CTGGGTAAGCCAAACATTGG	59 °C	[21]
12	hlyA-Forward	GGC AAA CAG CGA AAC AAA TAC C	62 °C	[22]
	hlyA-Reverse	CTC AGC GGG CTA ATA CGG TTT A	62 °C	[22]