(A) Representative immunoblots (left, bracket indicates identical gel) and quantification (right, normalized to GAPDH) for the indicated proteins, time points after TGFβ stimulation, and concentrations of MDL-28170 (n =3 to 4). (B) Representative immunofluorescence images of TGFβ-induced EMT in NMuMG cells stained with E-cadherin (green), F-actin (red), and DAPI (blue). Scale bar: 100 µm. (C) Relative gene expression (normalized to Gapdh) in response to TGFβ with or without calpain inhibition (MDL) (n = 3). (D) Representative immunoblots (left) and quantification (right) for the indicated proteins, time points after TGFβ stimulation, and concentrations of calpeptin (n = 3). (E) Representative immunoblots (left) and quantification (right) for the indicated proteins, time points after TGFβ stimulation, and concentrations the calcium channel blocker 2-APB (n = 3). Data are expressed as mean ± s.e.m. *P < 0.05, **P < 0.01, †P < 0.005, ‡P < 0.001 by one-way ANOVA with Tukey’s post hoc test.