(A) Genotype frequencies of embryos derived from Lipt1N44S/+ intercrosses.
(B) Lateral view of mouse embryos at embryonic day (E) 10.5 with the denoted Liptl genotypes. Scale bars: 1 mm.
(C) Immunoblot analysis of embryos with WT Liptl (Liptl+/+) or with heterozygosity {LiptTN44S/+) or homozygosity (Lipt1N44S/N44S) for the N44S variant. All embryos on the blot are from the same litter; these results are representative of two independent litters.
(D) PDH activity in mouse embryos with the denoted genotypes. The data are the mean and SD of Lipt1+/+ (n = 3) or Lipt1N44S/+ (n = 8) embryos. The data from two individual Lipt1N44S/N44S embryos are also shown. Unpaired two-tailed t tests were used to assess statistical significance. *p < 0.05; n.s., not significant.