TABLE 1.

Manufacturer 0.5-times–diluted reaction setup chart

Reagent	Volume (μl)
Dye Ready Reaction Mix	2
5× sequencing buffer	1
Primer 0.8 μM (1.6 pMol in reaction)	2
Water	4
Template	1
Reaction volume total	10

Cycling conditions	Seconds
96	60
96	10
50	5
60	240
25 cycles