TABLE 2.

Setup chart for difficult-to-sequence samples using optimal protocol for difficult templates

Reagent	Volume (μl)
Template (200 ng)	1
Primer (4 μM) 2 or 4 pmol	TBD
Dye (BD v3.1 or comparable)	1.5
dGTP v3.0	0.5
5× Buffer Sequencing Buffer	0
Additive (Betaine)	2
Water	TBD
Reaction volume total	10

Cycling conditions	Seconds
96	10
50	5
60	240
35 cycles

TBD, to be determined.