Secreted cytokines of hMSC seeded scaffolds with varying pore orientation. A cytokine assay was conducted on eluted media after 7 days from scaffolds with isotropic and anisotropic pores. Values of array intensity were measured with ImageJ, and a control array (exposed only to media) was used to determine fold change due to cellular activity. 5 key groups are shown – cytokines connected to inflammation, anti-inflammatory cytokines, cytokines connected to cell movement, angiogenic cytokines, bone formation cytokines. Green indicates cytokines with a higher fold change and yellow indicates limited or no change compared to media. * indicates a statistically significant (p < 0.05) increase in cytokine level for anisotropic pores over isotropic pores (power: 0.69176). Data expressed as average ± standard deviation (n = 3).