Figure 5.

ER stress increases stress response in G1 kidney organoids. (A) Real-time quantitative PCR of RNA isolated from whole-well G0 and G1 organoids reveals increased unfolded protein response (UPR) gene expression (EDEM1, HSPA5) in G1 organoids exposed to both IFN-γ and tunicamycin (P=0.01 by two-way ANOVA). (B) Heatmap depicts relative average expression of UPR-relevant genes for G0 and G1 glomerular epithelial cells subjected to either IFN-γ alone or both IFN-γ and tunicamycin. (C) Heatmap depicts relative average expression of apoptosis-relevant genes for G0 and G1 glomerular epithelial cells subjected to both IFN-γ and tunicamycin.