Concentrations of nitrite and cyclodextrin diffused across differentiated lung tissue at various timepoints and the percent tissue viability following a 6 h exposure to CD-PA/NO and CD-PA/NO^a.

Donor solutionb	Timepoint range (h)	[Nitrite] (μM)	[Nitrite]c (%)	[CD] (mg mL−1)	[CD]c (%)	Tissue viabilityd (%)
2.0 mg mL−1 CD-PA/NO	0–2	27.5 ± 1.3	4.7 ± 0.2	N. D.e	<0.65e	113.1 ± 9.0
	2–4	56.8 ± 1.3	7.3 ± 0.2	N. D.	<0.65
	4–6	95.8 ± 2.2	10.6 ± 0.2	0.026 ± 0.002*†	1.3 ± 0.1*†
2.0 mg mL−1 CD-PA	0–2	#	#	N. D.	<0.65	107.0 ± 8.9
	2–4	#	#	N. D.	<0.65
	4–6	0.2 ± 0.2	0.0 ± 0.0	0.071 ± 0.014†	3.5 ± 0.7†
2.0 mg mL−1 CD-PA/NO + 15 μL ASM	0–2	28.1 ± 1.3	4.8 ± 0.2	N. D.	<0.65	101.3 ± 5.3
	2–4	59.1 ± 1.7	7.6 ± 0.2	N. D.	<0.65
	4–6	98.6 ± 1.3	10.9 ± 0.1	0.020 ± 0.002*	1.0 ± 0.1*

^a N = 3 for all reported values.

^bAll donor solutions were made in PBS (pH 7.4) and 100 μL of the solutions were added to the apical side of the tissue.

^cPercentage of the nitrite or CD that diffused compared to the total NO released up to that time or initial concentration of CD in donor solution.

^dViability only measured following entire 6 h exposure.

^eValues were below the detection limit of our HPLC method which was <0.013 mg mL⁻¹ CD (or 0.65%). ^#Griess assay was not performed at these timepoints. *p < 0.01 compared to CD-PA. ^†p < 0.05 compared to CD-PA/NO + ASM.