Table 3.
Output data from Rest-RG software revealed the level of renal gene expression of Cu-Zn SOD, GPX, and TNF-α between control and UTD groups. The level of significant difference against the control group was set at P-value<0.05
| Gene | Type | Reaction efficiency | Expression | Std. Error | 95% C.I. | P(H1) | Result |
|---|---|---|---|---|---|---|---|
| β-actin | REF | 0.7663 | 1.000 | ||||
| Cu-Zn SOD | TRG | 0.7813 | 6.392 | 3.808 - 13.108 | 3.160 - 15.333 | 0.008 | UP |
| GPX | TRG | 0.7813 | 4.721 | 2.049 - 8.303 | 1.654 - 10.252 | 0.012 | UP |
| TNF-α | TRG | 0.8075 | 91.592 | 46.152 - 153.749 | 37.888 - 166.887 | 0.002 | UP |
P(H1): Probability of alternative hypothesis that difference between untreated diabetic and control groups is due only to chance
TRG: Target; REF: Reference β-actin: Beta-actin, (Cu-Zn) SOD: Cu-Zn Superoxide Dismutase, GPX: Glutathione Peroxidase, TNF-α: Tumor Necrosis Factor Alpha