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IL-4 assay in different immunized mice. (a) The effect of PLGA NP delivery system on IL-4, (b) the ratio of INF- ɣ to IL-4 after BCG priming and DNA vaccine boost regimen. All data were presented as mean±SD. (*P-value <0.05, ***P-value <0.001 compared to negative control). IL-4: Interleukin, IFN-γ: Interferon gamma, PLGA: Poly (lactic-co-glycolic acid), NP: Nanoparticle, BCG: Bacilli Calmette-Guérin
