Video 2. Droplet sorting in an anaerobic environment for human stool bacteria cultured in BHIS droplets for 1 day.

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The top left time stamp is in seconds. For each droplet, the region between the 2 red dots is analyzed using the Wavelet OD. For empty droplets and droplets containing a dense colony, the Wavelet OD does not satisfy the thresholding criteria (the decision is labeled as false, ‘F’) and the droplets flow down the waste path. When the threshold criteria are met for a sparse colony (the decision is labeled as true, ‘T’), the electrodes are actuated sending the droplet to the ‘keep’ path. We note that the spots in the oil phase were observed even for droplets generated without bacteria.