Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Anopheles gambiae) | AGAP001899 | NA | NCBI Gene ID: 1281226 |
|
| Gene (Anopheles gambiae) | AGAP003050 | NA | NCBI Gene ID: 1272902 |
|
| Genetic reagent (An. gambiae) | UAS-mCD8: mCherry responder line | doi: https://doi.org/10.1016/j.ibmb.2018.03.005 | NA | Transgenic line previously generated in Adolfi et al., 2018 and referred to as UAS-mCD8:mCherry line. Contains the coding sequence for the m-cherry fluorescent protein under the UAS promoter |
| Genetic reagent (An. gambiae) | Ubi-A10 Gal4 driver line | doi: https://doi.org/10.1016/j.ibmb.2018.03.005 | NA | Transgenic line previously generated in Adolfi et al., 2018
and referred to as PUBc-GAL4. Contains the coding sequence for the Gal4 transcription factor under the control of the An. gambiae Polyubiquitin-c (PUBc) gene |
| Genetic reagent (An. gambiae) | Oeno-Gal4 driver line | doi: https://doi.org/10.1101/742619 | NA | Transgenic line previously generated in Lynd et al., 2019 and referred to as A14Gal4. Contains the coding sequence for the Gal4 transcription factor under the control of an oenocyte specific enhancer |
| Genetic reagent (An. gambiae) | A11 docking line | doi: https://doi.org/10.1101/742619 | NA | Transgenic line previously generated in Lynd et al., 2019. It is carrying two AttP docking sites flanking 3xP3:eCFP marker gene. Used for phiC31 driven RMCE of UAS-inverted repeat contructs tagged with alternative fluorescent marker gene |
| Genetic reagent (An. gambiae) | UAS-FAS1899i Responder line | This paper | NA | Transgenic line generated in this study carrying inverted repeats targeting the FAS1899 gene under the control of the UAS promoter |
| Genetic reagent (An. gambiae) | UAS-Desat3050i Responder line |
This paper | NA | Transgenic line generated in this study carrying inverted repeats targeting the FAS1899 gene under the control of the UAS promoter (See Materials and methods section) |
| Recombinant DNA reagent | pSL*attB:YFP:Gyp:UAS14i:Gyp:attB (plasmid) | doi: https://doi.org/10.1101/742619 | Used to synthesise the inverted repeats for the generation of FAS1899i and Desat3050i transgenic lines (See Materials and methods section) | |
| Commercial assay or kit | Arcturus PicoPure RNA Isolation Kit | Thermo Fisher | Cat. #: KIT0204 |