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. 2020 Jul 2;11:847. doi: 10.3389/fphar.2020.00847

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Copyright © 2020 Shao, Shi, Li, Zhang, Pan, Fu and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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lincROR overexpression reversed Curcumin-induced growth inhibition and inactivated Wnt/β-catenin signaling. (A) The expression of lincROR in lincROR overexpressing SMMC-7721 cells was measured by qRT-PCR examination. ***P<0.001, compared with pVector. (B, C) The cell viabilities (B) and colony formation (C) was measured with Curcumin 48-hour treatment in lincROR overexpressing SMMC-7721 cells. (D) The protein level of β-catenin was examined by Western blotting after 48-hour treatment of Curcumin in lincROR overexpressing SMMC-7721 cells. (C) Other Wnt/β-catenin downstream target genes were examined by qRT-PCR with the same treatment in lincROR overexpressing SMMC-7721 cells. *P < 0.05; **P < 0.01; ***P < 0.001; compared with pVector+DMSO.