Fig 6.

The Attune worksheet for a sample of “sorted” URB4 microspheres. The sorting was performed using an Aria sorting flow cytometer. During sorting, only URB4 microspheres whose scattering signal fell in the gate associated with the major population were retained, all other microspheres were discarded. The scattering pattern in Gate R1(1) shows a tightly packed set of events, and none of the diffused events evident in Gate R1(1) in the previous Fig. 5. The data in Fig. 6 allows the measurement of the concentration of the microspheres in the major population. The fluorescence intensity from the sorted suspension will be due to only the major population.