KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-EspA-polyclonal rabbit | UT Southwestern, Vanessa Sperandio | N/A |
| anti-EspB-polyclonal rabbit | UT Southwestern, Vanessa Sperandio | N/A |
| goat-anti-rabbit-HRP | BioRad | Cat# 1706515 |
| Bacterial and Virus Strains | ||
| EHEC str 86-24 | U of Maryland, James Kaper | N/A |
| E. coli DH5α | UT Southwestern, Vanessa Sperandio | N/A |
| E. coli TOP10 | Invitrogen | Cat# C404052 |
| E. coli BL21 | Invitrogen | Cat# C600003 |
| Citrobacter rodentium DBS770 | Tufts University, John Leong | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 12 well Falcon plates | Fisher Scientific | Cat# 353043 |
| 12% Mini-PROTEAN TGX Stain Free protein gels | Biorad | Cat# 4568044 |
| 125 mL Polycarbonate Erlenmeyer Flask with Vent Cap | Corning | Cat# 431143 |
| 18mm coverslips | Fisher Scientific | Cat# 125461 |
| 1ml Disposable syringe | Becton Dickinson | Cat# 309602 |
| 5ml Disposable columns | Qiagen | Cat# 1018597 |
| 30% Acrylamide/Bis solution | Biorad | Cat# 161-0156 |
| 4–20% Mini-PROTEAN TGX Stain Free protein gels | Biorad | Cat# 4568094 |
| 3-indole acetonitrile | Sigma-Aldrich | Cat# 129453 |
| 3-indole acetic acid | Sigma-Aldrich | Cat# I2886 |
| 5-Hydroxyindole | Sigma-Aldrich | Cat# H31859 |
| Adenosine triphosphate | PerkinElmer | Cat# BLU002A250UC |
| Amicon ultra-15 centrifugal filter unit | Millipore | Cat# UFC901024 |
| Ammonium persulfate | Sigma-Aldrich | Cat# A3678-256 |
| Ampicillin powder | Sigma-Aldrich | Cat# A9518 |
| Antifade reagent | Life technologies | Cat# P36934 |
| Bio-Beads SM-2 Adsorbent media | Biorad | Cat# 152-8920 |
| Bio-Safe- Coomassie Stain | Biorad | Cat# 1610787 |
| Bovine Serum Albumin | Sigma-Aldrich | Cat# A2153 |
| Brain heart infusion agar | Becton Dickinson | Cat# 241810 |
| Calcium chloride | Sigma-Aldrich | Cat# C1016 |
| Chloramphenicol | Omnipur | Cat# 3130 |
| Chloroform | IBM Scientific | Cat# 67-66-3 |
| D-(+)- Glucose | Sigma-Aldrich | Cat# G8270 |
| Dithiothreitol (DTT) | Fisher Scientific | Cat# BP172-25 |
| DL-Histidine monohydrochloride monohydrate | Sigma-Aldrich | Cat# H7875 |
| dNTP mix 10mM | Invitrogen | Cat# 18-427-088 |
| Dulbecco eagle medium (DMEM) high glucose | Invitrogen | Cat# 11965-092 |
| Dulbecco eagle medium (DMEM) low glucose | Invitrogen | Cat# 11885-084 |
| E. coli polar lipid extract | Avanti polar lipids, Inc. | Cat# 100600 |
| Ethanol anhydrous | IBM Scientific | Cat# 64-17.5 |
| Ficoll solution | Sigma-Aldrich | Cat# F5415-25 |
| FITC-Phalloidin | Sigma-Aldrich | Cat# P5282 |
| Flouxetine hydrochloride | United States Pharmacopeia | Cat# 1279804 |
| Formaldehyde | Fisher Scientific | Cat# BP539 |
| Gibson assembly master mix | New England Biolabs | Cat# M5510A |
| Heat inactivated Fetal Bovine serum (FBS) | Thermo Fisher | Cat# 10082-147 |
| Horse blood serum | Colorado serum company | Cat# 31324 |
| Hydrochloric acid solution | Sigma-Aldrich | Cat# 318949-500 |
| Imidazole | Sigma-Aldrich | Cat# I2399 |
| Immun-Blot Low Fluorescence PVDF sets | Biorad | Cat# 1620260 |
| Indole | Sigma-Aldrich | Cat# I3408 |
| Isopropyl β-D-1-thiogalactopyranoside (IPTG) | Sigma-Aldrich | Cat# I6758 |
| Kanamycin sulfate powder | Sigma-Aldrich | Cat# K1876 |
| L-(+)-Arabinose | Sigma-Aldrich | Cat# A3256 |
| L-Cysteine HCl | Sigma-Aldrich | Cat# C1276 |
| L-Tryptophan | Sigma-Aldrich | Cat# T0254 |
| LB Agar, Miller | Fisher Scientific | Cat# BP1425 |
| LB broth, Miller | Fisher Scientific | Cat# BP9723 |
| Lysing matrix C, glass beads | MP biomedicals | Cat# 6912100 |
| Magensium chloride hexahydrate | Fisher Scientific | Cat# M35-500 |
| Magnesium acetate | Fisher Scientific | Cat# BP215-500 |
| Magnesium sulfate heptahydrate | Sigma-Aldrich | Cat# 230391 |
| Metronidazole | MP biomedicals | Cat# 0215571025 |
| n-Dodecyl β-D-maltoside | Sigma-Aldrich | Cat# D4641 |
| Neomycin trisulfate salt hydrate | Sigma-Aldrich | Cat# N5285 |
| Ni-NTA Agarose | Qiagen | Cat# 1018244 |
| N,N-Dimethyldodecylamine N-oxide | Sigma-Aldrich | Cat# 40234 |
| Octyl β-D-glucopyranoside | Sigma-Aldrich | Cat# O8001 |
| PCPA (4-Chloro-DL-phenylalanine) | Sigma-Aldrich | Cat# C6506 |
| Penicillin-Streptomycin solution | Thermo Fisher | Cat# 15140-122 |
| Phosphate Buffered Saline | Fisher Scientific | Cat# BP399-4 |
| Phusion High fidelity PCR master mix | Thermo Fisher | Cat# F-531L |
| Poly(2-deoxyinosinic2-deoxycytidylic acid) | Sigma-Aldrich | Cat# 81345-500 |
| Potassium phosphate dibasic | Fisher Scientific | Cat# BP363 |
| Potassium phosphate monobasic | Fisher Scientific | Cat# P286 |
| Precision Plus Protein Dual Color Standards | Biorad | Cat# 161-0374 |
| Propidium Iodine | Sigma-Aldrich | Cat# P4864 |
| Protease inhibitor cocktail | Sigma-Aldrich | Cat# P8849-5 |
| Proteinase K from Tritirachium album | Sigma-Aldrich | Cat# P2308 |
| Randon primers | Invitrogen | Cat# 48190-011 |
| Reusable animal feeding needle | Cadence science | Cat# 7920 |
| Reverse transcriptase | Thermo Fisher | Cat# 4308228 |
| Ritanserin | Sigma | Cat# R103 |
| RnaseA inhibitor | Thermo Fisher | Cat# 100021540 |
| RNaseA powder | Fisher Scientific | Cat# BP23391 |
| Serotonin | Sigma | Cat# 14927 |
| Serotonin hydrochloride | Tocris | Cat# 3547 |
| Sodium chloride | Sigma-Aldrich | Cat# S9888-1 |
| Sodium hydroxide solution 10N | Millipore | Cat# SX0607N-6 |
| Steriflip-GP sterile centrifuge tube top filter unit | Millipore | Cat# SCGP00525 |
| Streptomycin powder | Sigma-Aldrich | Cat# S6501 |
| Super script II reverse transcriptase | Invitrogen | Cat# 18064-014 |
| SuperSignal West Dura Extended Duration Substrate | Thermo Fisher | Cat# 34075 |
| Sybr green master mix | Thermo Fisher | Cat# 4309155 |
| T4 DNA Ligase | New england Biolabs | Cat# MO202L |
| T4 polynucleotide kinase | New england Biolabs | Cat# M0201S |
| TEMED | Biorad | Cat# 161-0801 |
| Terminal Deoxynucleotidyl transferase | Promega | Cat# M1875 |
| TrackIt 1 Kb Plus DNA Ladder | Thermo Fisher | Cat# 10488085 |
| Trans-Blot Turbo Mini PVDF Transfer Packs | Biorad | Cat# 1704156 |
| Tris base | Fisher Scientific | Cat# BP152-10 |
| Tritiated serotonin | Perkin Elmer | Cat# NET498001MC |
| Tritiated tryptophan | Perkin Elmer | Cat# NEC367010UC |
| Triton X-100 | Fisher Scientific | Cat# BP151 |
| Trizol reagent | Thermo Fisher | Cat# 1559618 |
| Tryptamine | Sigma-Aldrich | Cat# 193747 |
| Tryptone | Fisher Scientific | Cat# BP1421-2 |
| Tryptophan | Sigma-Aldrich | Cat# T0254 |
| Tween 20 | Fisher Scientific | Cat# BP337-500 |
| Vancomycin hydrochloride | Research Products International | Cat# V06500 |
| Yeast Extract | Fisher Scientific | Cat# BP1422-2 |
| Zicronium beadsBeadBug prefilled tubes | Sigma-Aldrich | Cat# Z763802 |
| Critical Commercial Assays | ||
| Rnasy power microbiome | Qiagen | Cat# 26000-50 |
| Ribopure-bacteria | Ambion | Cat# 1405072 |
| Genelute Plasmid Miniprep | Sigma | Cat# PLN350-1KT |
| QIAquick PCR purification kit | Qiagen | Cat# 28906 |
| QIAquick gel ectraction kit | Qiagen | Cat# 28704 |
| Illumina ScriptSeq Complete Kit | Illumina | BB1224 |
| Deposited Data | ||
| RNA sequencing data | GEO GSE144795 | GEO GSE144795 |
| Experimental Models: Cell Lines | ||
| HeLa cell line | ATCC | Cat# ATCC® CCL-2.1 |
| Experimental Models: Organisms/Strains | ||
| Mouse C57BL/6J mice | The Jackson laboratory | Cat# 000664 |
| B6.129(Cg)-Slc6a4 tm1Kpl/J | The Jackson laboratory | Cat# 008355|5-HTT- |
| Oligonucleotides | ||
| Primers used in this study, see Table S2 | This paper | N/A |
| Software and Algorithms | ||
| GraphPad Prism 7 for Mac | https://www.graphpad.com/ | N/A |
| Clustal Omega | https://www.ebi.ac.uk/Tools/msa/clustalo/ | N/A |
| DNASTAR Lasergene software version 15.0.0 | DNASTAR, Inc. | N/A |
| Fiji | https://fiji.sc | doi:10.1038/nmeth.2019 |
| Image Lab software version 5.2.1 | Biorad | N/A |