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. Author manuscript; available in PMC: 2021 Jul 8.
Published in final edited form as: Cell Host Microbe. 2020 Apr 22;28(1):23–30.e5. doi: 10.1016/j.chom.2020.04.002

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
rabbit anti-FLAG Sigma-Aldrich Cat# F7425; RRID: AB_439687
mouse anti-FLAG Sigma-Aldrich Cat# F1804; RRID: AB_262044
HRP-conjugated goat anti-Rabbit IgG Bio-Rad Cat# 170-6515; RRID: AB_11125142
HRP-conjugated goat anti-mouse IgG Santa Cruz Biotechnology Cat# sc-2005; RRID: AB_631736
Bacterial and Virus Strains
Listeria monocytogenes 10403s Rauch et al., 2017 RefSeq: NC_017544.1
Listeria monocytogenes 10403s derivatives This paper See Table S2
Pseudomonas aeruginosa strain PAO1 Laboratory of Alan Davidson RefSeq: NC_002516.2
Pseudomonas aeruginosa strain PAO1 derivatives This paper N/A
Escherichia coli DH5α New England Biolabs Cat #C2982I
Escherichia coli SM10 Laboratory of Daniel Portnoy N/A
Listeria phage A006 This paper RefSeq: NC_009815.1
Listeria phage A006 derivatives This paper See Table S2
Listeria phage A118 This paper RefSeq: NC_003216.1
Listeria phage A502 This paper RefSeq: MDRA00000000
Listeria phage A620 This paper N/A
Listeria phage J0161a Rauch et al., 2017 RefSeq: NC_017545.1
Listeria phage J0161a derivatives This paper N/A
Listeria phages P35 This paper RefSeq: NC_009814.1
Pseudomonas phage JBD30 Laboratory of Alan Davidson RefSeq: NC_020198.1
Chemicals, Peptides, and Recombinant Proteins
AcrIIA1 protein homologs tested for promoter repression This paper See Table S1
Purified protein: AcrIIA1 This paper N/A
Monolith His-Tag Labeling Kit RED-tris-NTA Nanotemper Technologies Cat #MO-L018
Tetrazolium Violet TCI Chemicals Cat #T0174
Critical Commercial Assays
Gibson Assembly Master Mix New England Biolabs Cat #E2611 L
Phusion Hot Start Flex DNA Polymerase New England Biolabs Cat #M0535S
Oligonucleotides
Listeria reporter phage lysogen confirmation Primer1: TAATTTGCTTAACTGATACC This paper N/A
Listeria reporter phage lysogen confirmation Primer2: TGACTACTACGTATATTCG This paper N/A
Wild-type Acr promoter for in vitro binding assay: AACTATTGACTACTACGTATATTCGTAGTATAATGTGAAT This paper N/A
Terminal Mutations Acr promoter for in vitro binding assay: AACTATTGACAACTACGTATATTCGTAGTTTAATGTGAAT This paper N/A
Six Mutations Acr promoter for in vitro binding assay: AACTATTGACAACAACCTATATTGGTTGTTTAATGTGAAT This paper N/A
Recombinant DNA
AcrIIA1-associated promoter sequences Twist Bioscience See Table S1
pKSV7 Rauch et al., 2017 addgene.org/26686/
pKSV7-derivative plasmids This paper See Table S2
pPL2oexL Rauch et al., 2017 https://doi.org/10.1016/j.cell.2016.12.009
pPL2oexL-derivative plasmids This paper See Table S2
pLEB579 Beasley et al., 2004 https://doi.org/10.1093/ps/83.1.45
pLEB579-derivative plasmids This paper See Table S2
pHERD30T Laboratory of Alan Davidson GenBank: EU603326.1
pHERD30T-derivative plasmids This paper N/A
pMMB67HE ATCC http://www.snapgene.com/resources/plasmid_files/basic_cloning_vectors/pMMB67HE/
pMMB67HE-derivative plasmids This paper N/A
pET28 protein expression plasmid Laboratory of David Morgan N/A
pET28-6xHis-AcrIIA1 protein expression plasmid This paper N/A
Software and Algorithms
Prism 6.0 GraphPad https://www.graphpad.com/scientific-software/prism/
Gen 5 BioTek https://www.biotek.com/products/software-robotics-software/gen5-microplate-reader-and-imager-software/
Image Lab 5.2.1 BioRad http://bio-rad.com/en-cn/product/image-lab-software
NanoTemper Analysis Software NanoTemper Technologies https://nanotempertech.com/monolith/
Other
Synergy H1 Microplate Reader BioTek https://www.biotek.com/products/detection-hybrid-technology-multi-mode-microplate-readers/synergy-h1-hybrid-multi-mode-reader/
Azure c600 Imager Azure Biosystems https://www.azurebiosystems.com/imaging-systems/azure-600/
Monolith NT.115 NanoTemper Technologies https://nanotempertech.com/monolith/