Fig. 1. Cytokine expression by Ly6Chi monocytes during murine hepatic amebiasis.
a Representative T2-weighted MRI images of male and female mouse liver tissues infected with E. histolytica on Days 3, 5, and 7 p.i. b Abscess volume in male and female mice during the course of infection (nf = 13, nm = 14). Plasma concentration of c TNF, d CCL2, and e CXCL1 in male and female mice during the course of disease (c nm/f d0 = 6/8; nm/f d2 = 8/8; nm/f d4 = 8/7; nm/f d6 = 8/8; d nm/f d0 = 6/8; nm/f d2 = 8/8; nm/f d4 = 8/8; nm/f d6 = 8/8; e nm/f d0 = 7/7; nm/f d2 = 7/6; nm/f d4 = 7/6; nm/f d6 = 7/6; pooled data from two independent experiments; Depicted are the means ± SEM). f Representative gating strategy used to identify murine CD11bneg, Ly6Cneg, Ly6G+, Ly6Clo, and Ly6Chi subpopulations by flow cytometry. g Intracellular expression of TNF, CCL2, and CXCL1 by murine liver-derived cell subpopulations identified in f on Day 3 p.i. (nLy6Chi, Ly6Clo, Ly6Cneg, CD11bneg = 12; nLy6G+ = 6, male and female mice; pooled data of two independent experiments; Depicted are box ranging from 25th to 75th percentile and whiskers from min to max including the median). Intracellular staining of h TNF, i CCL2, and j CXCL1 in liver-derived Ly6Chi monocytes from naïve and E. histolytica-infected male and female mice on Day 3 p.i. (h nm/f d0 = 7/8; nm/f d3 = 7/5; i nm/f d0 = 7/8; nm/f d3 = 7/5; j nm/f d0 = 7/8; nm/f d3 = 7/5; pooled data of two independent experiments; Depicted are the means). k Abscess volume in αCXCL1-treated and E. histolytica-infected male mice on Day 3 p.i. (nαCXCL1 = 5; nctrl IgG = 7; pooled data of two independent experiments; Depicted are the means ± SEM). l Graph showing the percentage of Ly6Chi monocytes within the total liver-derived Ly6C+ monocyte population within CD11b+ cells from αCXCL1-treated and E. histolytica-infected male mice on Day 3 p.i. (nαCXCL1 = 5; nctrl IgG = 7; pooled data of two independent experiments; depicted are the means). P-values were calculated using two-tailed grouped analysis: Student’s t-test (h, j, l) or the Mann–Whitney test (b–e, g, k), *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). Source data are provided as a Source Data file.