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. 2020 Jul 10;11:3459. doi: 10.1038/s41467-020-17260-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Mice were either gonadectomized (G) or gonadectomized (4 weeks before infection, 8 weeks of age) and substituted with testosterone (T) (2 weeks before infection, 10 weeks of age). b Intracellular expression of TNF, CCL2, and CXCL1 by liver-derived Ly6C^hi monocytes from G and T male mice 3 days following intrahepatic E. histolytica infection, as analyzed by flow cytometry. (TNF: n_G/T = 7/9; CCL2: n_G/T = 7/9, CXCL1: n_G/T = 7/9; one experiment; depicted are the means). c Percentage of Ly6C^hi monocytes in the blood and liver of naïve or E. histolytica-infected G and T male mice (blood: n_{wt/G/T d0} = 16/14/9; n_{G/T d3} = 16/9; liver: n_{G/T d3} = 16/9; one experiment; depicted are the means). Percentage of d CD86⁺ cells and e MFI of CCR2 and f CX₃CR1 Ly6C^hi monocytes in the blood and liver of naïve or E. histolytica-infected G and T male mice (d blood: n_{wt/G/T d0} = 16/14/9; n_{G/T d3} = 16/9; liver: n_{G/T d3} = 16/9; e blood: n_{wt/G/T d0} = 16/14/9; n_{G/T d3} = 16/9; liver: n_{G/T d3} = 16/9; f blood: n_{wt/G/T d0} = 16/14/9; n_{G/T d3} = 16/9; liver: n_{G/T d3} = 16/9; one experiment; depicted are the means). g qPCR analysis of androgen receptor (AR) expression of RNA isolated from PBMCs of naïve sham operated or gonadectomized mice (n_sham/G = 12; one experiment; depicted are box ranging from 25th to 75th percentile and whiskers from min to max including the median). h qPCR analysis of androgen receptor (AR) expression of RNA isolated from PBMCs of E. histolytica-infected G and T mice (n_G/T = 17/9; pooled data from two independent experiments; depicted are box ranging from 25th to 75th percentile and whiskers from min to max including the median). P-values were calculated using two-tailed grouped analysis: Student’s t-test (b) or Mann–Whitney test (c, d, e–h), *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Source data are provided as a Source Data file.