Table 1.
In vitro cytotoxic activity of starting material compounds and indirubin-3′-oxime derivatives 6a–p. Indirubin-3′-oxime (compound 2) and Ellipticine was used as positive control.
| No. | Compounds | IC50 (μM) | ||||
|---|---|---|---|---|---|---|
| HepG2 | LU-1 | SW480 | HL-60 | HEK-293 | ||
| 1 | 1 | > 20 | > 20 | > 20 | > 20 | > 20 |
| 2 | 3 | > 20 | > 20 | > 20 | > 20 | > 20 |
| 3 | 4 | > 20 | 19.55 ± 1.23 | 18.59 ± 0.91 | 15.27 ± 0.35 | 16.22 ± 0.33 |
| 4 | 2 | 16.00 ± 0.62 | 16.36 ± 1.05 | 15.65 ± 0.74 | 16.50 ± 0.94 | 14.16 ± 0.71 |
| 5 | 6a | 3.56 ± 0.75 | 2.26 ± 0.32 | 3.36 ± 0.64 | 2.39 ± 0.46 | 3.21 ± 0.43 |
| 6 | 6b | > 20 | > 20 | > 20 | > 20 | > 20 |
| 7 | 6c | 3.38 ± 0.68 | 2.99 ± 0.55 | 3.8 ± 0.52 | 1.43 ± 0.22 | 1.19 ± 0.12 |
| 8 | 6d | 3.76 ± 0.75 | 2.85 ± 0.74 | 4.46 ± 1.18 | 3.40 ± 0.76 | 2.01 ± 0.64 |
| 9 | 6e | 4.62 ± 1.07 | 3.28 ± 0.48 | 4.66 ± 0.92 | 3.27 ± 0.37 | 6.98 ± 0.25 |
| 10 | 6f | 2.01 ± 0.43 | 1.30 ± 0.14 | 2.54 ± 0.25 | 0.98 ± 0.12 | 1.03 ± 0.11 |
| 11 | 6g | 5.68 ± 0.71 | 3.96 ± 0.62 | 4.10 ± 0.57 | 4.82 ± 0.46 | 1.80 ± 0.21 |
| 12 | 6h | 14.23 ± 1.79 | 8.74 ± 0.55 | 14.13 ± 1.18 | 6.50 ± 0.98 | 9.74 ± 0.53 |
| 13 | 6i | 2.28 ± 0.12 | 2.07 ± 0.26 | 3.43 ± 0.84 | 1.28 ± 0.34 | 0.85 ± 0.05 |
| 14 | 6k | 2.95 ± 0.19 | 2.50 ± 0.34 | 2.91 ± 0.13 | 1.29 ± 0.14 | 1.92 ± 0.17 |
| 15 | 6l | 3.28 ± 0.25 | 2.07 ± 0.31 | 3.03 ± 0.11 | 1.28 ± 0.16 | 7.12 ± 0.18 |
| 16 | 6m | 15.31 ± 0.17 | 8.74 ± 1.06 | 14.36 ± 0.72 | 15.96 ± 0.38 | 11.93 ± 1.75 |
| 17 | 6n | 11.70 ± 0.23 | 10.08 ± 0.44 | 12.54 ± 0.53 | 10.44 ± 0.84 | 9.52 ± 0.07 |
| 18 | 6o | 11.68 ± 0.26 | 8.80 ± 0.38 | 11.16 ± 0.76 | 9.39 ± 0.45 | 5.89 ± 0.75 |
| 19 | 6p | 10.61 ± 0.79 | 8.61 ± 0.57 | 11.71 ± 0.48 | 11.25 ± 1.04 | 7.72 ± 0.42 |
| 20 | Ellipticine | 1.93 ± 0.54 | 2.50 ± 0.82 | 1.76 ± 0.63 | 2.19 ± 1.12 | 0.32 ± 0.03 |