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. 2020 Jul 10;10:11415. doi: 10.1038/s41598-020-68040-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNA-seq identifies GR-dependent and GR-independent genes and effects of chronic cortisol treatment. (A) Diagram of experimental design using Visual Background Adaptation (VBA) selection. (B) Principal component plot of the RNA-seq data, showing the location of each replicate sample with respect to the first two principal components. Cortisol-treated samples are shown in red, vehicle-treated controls in black. VBA+ samples (containing at least one functional GR allele) are filled shapes, VBA- samples are empty shapes. (C) Venn diagram showing numbers of genes upregulated in each of the indicated comparisons (keyed as in (A)): VBA+ vs. VBA- (vehicle-treated controls); cortisol-treated vs. vehicle-treated VBA+; and cortisol-treated vs. vehicle treated VBA-. Upregulated genes in common respectively between the first two and the last two comparisons are listed on the right. (D) Box plots of Z-transformed expression levels of klf9 and npas4a obtained from the RNA-seq data. Sample numbers correspond to those in (B). Asterisks indicate significant differential expression (adjusted p values): ***< .0001; *< .05.