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. 2020 Jul 10;10:11415. doi: 10.1038/s41598-020-68040-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNA-seq identifies klf9-dependent transcriptomic effects of chronic cortisol exposure. (A) Scatter plot comparing differential gene expression in response to chronic cortisol treatment in wild type (WT) and klf9^-/- larvae. (B) Venn diagram comparing numbers of genes upregulated by cortisol treatment in wild type and klf9^-/- larvae, and overlap. (C) Examples of two of the genes upregulated by chronic cortisol in a klf9-dependent fashion that were also identified in our previous analysis. (D) Treemap generated by REVIGO⁵⁷ (https://revigo.irb.hr/) of gene ontology biological process terms found by GOrilla³⁴ to be enriched in the set of 408 genes upregulated by chronic cortisol in a klf9-dependent way.