Table 2.

Analysis of InDel wtPTE variants with at least 2-fold improved arylesterase activity.

		Activity fold change relative to wtPTEa				Location of mutationsb
		Average effect	Median	Maximum	Minimum	Total	Loop 7	Loop 8	Other
InDels	Paraoxon	0.16	0.21	1.5	<0.01	81	58	15	8
	4-NPB	3.0	2.8	14.4	2.0	56	50	1	5
	2-NH	7.4	6.4	138.6	2.6	25	8	14	3
Deletions	Paraoxon	0.08	0.15	0.86	<0.01	26	17	4	5
	4-NPB	2.7	2.4	5.2	2.0	16	13	0	3
	2-NH	5.3	5.2	10.5	2.6	10	4	4	2
Insertions	Paraoxon	0.22	0.28	1.5	<0.01	55	41	4	10
	4-NPB	3.2	2.9	14.4	2.1	40	37	1	2
	2-NH	9.5	8.1	138.6	3	15	4	10	1

^aValues refer to the activity change of all or AE positive variants relative to wtPTE obtained by comparing the initial rates v₀ for the hydrolysis of paraoxon, 4-NPB or 2-NH to that of wtPTE at 200 μM substrate concentration, resulting in a dimensionless ratio. The average effect value was determined as the geometric mean of the relative activities of all the variants listed in Supplementary Table S13. The maximum, median and minimum changes correspond to the maximum, median and minimum relative activities for each substrate among the variants (see also Supplementary Fig. S13).

^bThe values refer to the number of insertions and/or deletions observed in the entire sequence of wtPTE or in specific regions (e.g., loop 7 (residues 252–278) and loop 8 (residues 299–313)).