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. 2020 Jul 10;11(7):522. doi: 10.1038/s41419-020-2729-0

Fig. 3. Primary GC cells confirmed the anticancer effect by LINC01446 silencing.

Fig. 3

a GC tissues from two patients were obtained from the hospital, we isolated primary GC cells by collagenase digestion. b MTT assays for the si-LINC01446- or si-NC-transfected primary GC cells from patient 1 and patient 2. c The migratory abilities of LINC01446-silenced and control cells were detected using transwell assay. Scale bar: 120 μm. d P15, P16, P21, p27, KLF2, FN1, and Vimentin mRNA expression levels were determined by qRT-PCR following LINC01446 knockdown. Data were shown as mean ± SD, n = 3. Student’s t test, *P < 0.05. n.s. not significant.