Table 1.

Contributions of the CG and AT base pairs to the enthalpy (ΔH), entropy (ΔS), Gibbs energy (ΔG = ΔH − TΔS) and the heat capacity increment (ΔCp) on DNA duplex dissociation at 25 °C in 150 mM NaCl, 5 mM phosphate, pH 7.4, aqueous solution

Base pair	ΔHcoop (kJ/mol-bp)	ΔScoop (J/(K mol-bp)	ΔGcoop (kJ/mol-bp)	ΔCp [kJ/(K mol-bp)]
CG	19.0 ± 0.3	36.2 ± 0.2	8.2 ± 0.2	0.13 ± 0.01
AT	28.0 ± 0.3	73.5 ± 0.5	6.1 ± 0.2	0.13 ± 0.01

To minimize the near-neighbor effect, DNA duplexes containing at least 12 base pair of contiguous CG or AT sequences were chosen (Vaitiekunas et al. 2015). All data have been corrected for the translation entropy to exclude the effects of duplex concentration (Privalov and Crane-Robinson 2018b). These two precautions permitted a significant decrease in the error in estimating the thermodynamic contributions of the base pairs