Fig. 6.

Pre-treatment with BSO (50 µM), an inhibitor of GSH synthesis, reduced the beneficial effects of δ-TT against the excessive generation of intracellular ROS stimulated by t-BHP in MC3T3-E1 cells. The cells were treated with the inhibitor 24 h before receiving δ-TT (10 µg/ml) and t-BHP (250 μM) treatments. The intracellular ROS levels were measured 30 min after t-BHP using CM-DCFA assay. Data are the mean ± SEM of six replicates within a single experiment. *p < 0.01, ***p < 0.001 vs controls; °°p < 0.01 vs BSO