Fig. 7.

Effects of pre-treatment with a the selective inhibitor of sirtuin 1 (EX-527, 25 µM); b farnesylpyrophosphate (FPP, 5 µM) or geranylgeranylpyrophosphate (GGPP, 5 µM) on the δ-TT protective effect against t-BHP-induced MC3T3-E1 cytotoxicity. The cells were treated with each compound 2 h before receiving δ-TT (10 µg/ml) and t-BHP (250 μM for 3 h) treatments. Data are the mean ± SEM of six replicates within a single experiment. ***p < 0.001 vs controls; °°°p < 0.001 vs t-BHP