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. 2020 Jul 10;3:368. doi: 10.1038/s42003-020-1063-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Confocal images showing the relative localization of VAMP7 (red) labelled with anti-Flag Abs and Giantin (green) in VAMP7-KO Jurkat cells expressing the WT VAMP7-FLAG or the mutant VAMP7-C183A-FLAG proteins. Scale bar 5 μm. b Dot plots showing the quantification of the percentage of Giantin (Golgi marker) overlapping with the VAMP7-FLAG proteins (Manderson coefficient). Each dot = one cell; horizontal lines = median. ****p < 0.0001 (one-way ANOVA). c Representative confocal images of conjugates of Jurkat VAMP7-KO cells expressing the WT VAMP7-FLAG or the mutant VAMP7-C183A-FLAG proteins and SEE-pulsed Raji B cells (blue) labeled with anti-FLAG (VAMP7-FLAG, showed in red) or anti-Giantin (shown in green) antibodies, assessed at 15 min. Scale bar 5 μm. d Mapping and e quantification of the enrichment of VAMP7 at the immune synapse (depicted by the dotted white line) in Jurkat “mean cells” interacting for 15 min with Raji cells left unpulsed (−, inactivated state) or pulsed with SEE (+, immune synapse formation). N number of cells constituting the mean image. Horizontal lines represent median. Scale bar 5 μm. Each dot = one cell; horizontal lines = median. ****p < 0.0001 (t-test and one-way ANOVA). Data are from two independent experiments.