Fig. 3. Various types of cells imaged by the RIACS.

Processing of the raw images was performed using ImageJ. Scale bars, 10 µm. a SRS images of various microalgal cells whose size ranges from 3 to 20 µm in cell diameter (n = 10,348 for Chlorella sorokiniana cells, n = 12,236 for Chlamydomonas reinhardtii cells, n = 11,050 for Hamakko caudatus cells, and n = 12,793 for Gloeomonas anomalipyrenoides cells). b SRS images of 3T3-L1 cells that gradually accumulated lipids in the cytoplasm over 7 days of treatment for inducing their differentiation into adipocyte-like cells (n = 11,159 for cells without treatment and n = 5,892, 10,359, and 10,114 for cells with 4, 5, and 7 days of treatment, respectively). c SRS images of Euglena gracilis cells with ¹²C/¹³C-isotope probing (n = 5679 and 2075, respectively). d SRS images of hiPSCs cultivated in two different culture media for the naïve pluripotent state (with 2 days of treatment, n = 1699) and the primed pluripotent state (without treatment, n = 1641).