Fig. 4. Raman image-activated sorting of various types of cells with the RIACS.

a Procedure for sorting adipocyte-like cells. b Scatter plot of differentiated and undifferentiated 3T3-L1 cells in the spatial distribution of intracellular lipid droplets and the lipid intensity per cell area. 3T3-L1-derived adipocyte-like cells with a large spatial distribution of cytoplasmic lipid droplets (indicated by the yellow region) were sorted by the RIACS. The insets show representative SRS images of cells in the sort (n = 108) and unsort regions (n = 11,068). Scale bar: 10 µm. c Procedure for sorting Chlamydomonas sp. cells. d Scatter plot of Chlamydomonas sp. mutant cells in cell area and lipid density. Highly lipid-rich, but rare (only 0.3% of the total population) Chlamydomonas sp. mutants (indicated by the yellow region) were sorted by the RIACS. The inset shows representative SRS images of cells in the sort (n = 26) and unsort regions (n = 7760). Scale bar: 10 µm. e Procedure for sorting Euglena gracilis cells. f Scatter plot of the 50/50 mixture of Euglena gracilis cells cultured in NaH¹²CO₃ and NaH¹³CO₃ in ¹²C- and ¹³C-paramylon intensity per cell area. Euglena gracilis cells cultured in NaH¹³CO₃ (indicated by the yellow region) were sorted by the RIACS. The inset shows representative SRS images of cells in the sort (n = 2075) and unsort regions (n = 13,669). Scale bar: 10 µm.