Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 18;9(3):21. doi: 10.1167/tvst.9.3.21

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2020 The Authors

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

PMC Copyright notice

Figure 5. — Retinal development is recapitulated in the differentiating PGP1 hiPSC-derived retinal organoids. (A–C) Undifferentiated PGP1 hiPSCs day 0 (D0, blue) and differentiated PGP1 retinal organoids at day 55 (D55, black) were used to measure mRNA levels of various markers via RT-qPCR normalized to GAPDH. (A) Expression of stem cell markers OCT4, NANOG; (B) NRP markers PAX6,SIX3,LHX2; (C) Retinal pigment epithelium (RPE) markers MITF, BEST1. (D–F) PGP1 hiPSCs at D0 and hiPSCs retinal organoids at D55, D95, and D166 of differentiation were used to measure mRNA levels via RT-qPCR normalized to GAPDH. (D) NRP and bipolar marker VSX2, (E) RGC marker BRN3b, and (F) PR marker RCVRN. As organoid differentiation progressed from D0 to D55, stem cell markers significantly decreased and markers of retina and RPE differentiation significantly increased. Compared to D55, the PR marker RCVRN progressively increased in its mRNA expression, whereas the NRP marker VSX2 and the RGC marker BRN3b gradually decreased in the older retinal organoids. Error bars represent standard error of the mean (SE), paired t test. *P < 0.05. **P < 0.005. ***P < 0.0005. Primer information is listed in Supplementary Table S5.