Table 1.

Primer sequences and PCR fragment sizes used for virus and bacterial identification in this study.

Pathogen target	Primer sequence(5′-3′)	Fragment size	Reference
PRRSV	F: TGAYGGGCGACAATGTCC	319 bp	[6]
	R: CGCAGACAAATCCAGAVG
PEDV	F:TTCTGAGTCACGAACAGCCA	651 bp	[7]
	R: CATATGCAGCCTGCTCTGAA
TGEV	F: GTGGTTTTGGTYRTAAATGC	859 bp	[7]
	R: CACTAACCAACGTGGARCTA
PARV	F: AAAGATGCTAGGGACAAAATTG	308 bp	[8]
	R: TTCAGATTGTGGAGCTATTCCA
CSFV	F: AGACGGCCTGTACCATAATA	610 bp	[9]
	R: GTATAAGATGTCCACGG
PCV2	F: GAAGAATGGAAGAAGCGG	360 bp	[10]
	R: CTCACAGCAGTAGACAGGT
PRV	F: GGTGGACCGGCTGCTGAACGA	455 bp	[11]
	R: GCTGCTGGTAGAACGGCGTCA
PPV	F: AAATGAATCTGGGGGTGGGG	316 bp	[12]
	R: CCAGTCCGCTGGATTGAACC
Bacterial 16s rDNA	27F: AGAGTTTGATCCTGGCTCAG	1466 bp	[13]
	1492R:TACGGTTACCTTGTTACGACTT