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Differential expression level and m6A levels of AFF4 between CSCs and non-CSCs of BCa. mRNA levels (a) and protein levels (b) of AFF4 in ALDH1-positive and ALDH1-negative BCa cells. m6A modification in specific regions of AFF4 transcripts in ALDH1-positive and ALDH1-negative 5637 (c) and UM-UC-3 (d) cells was tested by gene-specific m6A-qPCR assay. ∗p < 0.05, ∗∗p < 0.01 compared to the scramble group, by Student t-test.
