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. 2020 Jul 2;2020:8704896. doi: 10.1155/2020/8704896

Figure 6.

Figure 6

Effect of the TLR4 inhibitor TAK-242 on the gene expression levels of IL-6, IL-8, and MCP-1 in the hPDLSC response to different LPS preparations. Primary hPDLSCs were stimulated with commercially available “standard” P. gingivalis LPS (1 μg/ml), “ultrapure” P. gingivalis LPS (1 μg/ml), and “ultrapure” E. coli LPS (1 μg/ml) in the presence (250 ng/ml) of sCD14. Additionally, in the appropriate groups, TLR4 inhibitor TAK-242 (5 μM) was added for 24 h. The resulting gene expression levels of IL-6, IL-8, and MCP-1 were measured by qPCR. The y-axis represents the n-fold expression levels of target gene in relation to unstimulated cells (n = 1). Data are presented as mean ± s.e.m. of five different donors. ∗Significantly different vs. control; #significantly different vs. stimulation in the absence of TAK-242.