Table 1.
Fluorescence quantification primer sequence.
| Gene | Primer sequencing (forward and reverse) | |
|---|---|---|
| TNF-α | Forward | 5′-TGGCAAATGTGAGAAACGAG-3′ |
| Reverse | 5′-AAACCAGAACAGACCCAACG-3′ | |
|
| ||
| IL-1β | Forward | 5′-TCCAGGATGAGGACATGAGCAC-3′ |
| Reverse | 5′-GAACGTCACACACCAGCAGGTTA-3′ | |
|
| ||
| IL-6 | Forward | 5′-CCACTTCACAAGTCGGAGGCTTA-3′ |
| Reverse | 5′-CCAGTTTGGTAGCATCCATCATTTC-3′ | |
|
| ||
| IDE | Forward | 5′-TCCCATACCAGACCTTCAGC-3′ |
| Reverse | 5′-GTATTCACCCAGCCCTTTGA-3′ | |
|
| ||
| FAS | Forward | 5′-ACTGCGATTCTTCTGGCTGT-3′ |
| Reverse | 5′-GCGATTTCTGGGACTTTGTT-3′ | |
|
| ||
| SREBP-1 | Forward | 5′-AACCAGAAGCTCAAGCAGGA-3′ |
| Reverse | 5′-TCATGCCCTCCATAGACACA-3′ | |
|
| ||
| Mcp-1 | Forward | 5′-CTGTGCTGACCCCAATAAGGA-3′ |
| Reverse | 5′-ACAGAAGTGCTTGAGGTGGT-3′ | |
|
| ||
| HNF-4α | Forward | 5′-AAATGTGCAGGTGTTGACCA-3′ |
| Reverse | 5′-CACGCTCCTCCTGAAGAATC-3′ | |
|
| ||
| β-actin | Forward | 5′-GTGCTATGTTGCTCTAGACTTCG-3′ |
| Reverse | 5′-ATGCCACAGGATTCCATACC-3′ | |