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. 2020 Jun 25;21(12):4539. doi: 10.3390/ijms21124539

Figure 1.

Figure 1

PCI-34051 has no effect on NO2- production or iNOS expression, whereas VPA significantly increases NO2− production after LPS stimulation. (A) BMDMs were stimulated with LPS for 1 h and then treated with VPA at 1000 µM and 2000 µM as a control and with PCI-34051 at 5 µM and 10 µM for 24 h. This experimental set-up was applied for all in vitro experiments. (B) Culture medium was collected to analyze the NO2− production via a Griess assay. VPA (1000 µM) significantly increased NO2− production after LPS stimulation. PCI-34051 does not affect nitrite production after LPS stimulation. (C,D) Total protein lysates were analyzed using Western blot analysis to examine the iNOS protein expression. VPA and PCI-34051 had no effect on iNOS production upon LPS stimulation. Representative blots are shown in d. Data are represented as relative values compared with control + LPS ± standard error of the mean (SEM); * p < 0.05; n = 3–4 independent experiments; BMDMs: bone marrow-derived macrophages; LPS: lipopolysaccharide; NO2−: nitrite; iNOS: nitric oxide synthase; C: vehicle control; VPA: valproic acid; PCI: PCI-34051.