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. 2020 Jun 10;12(6):1742. doi: 10.3390/nu12061742

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of hispolon on (A) ER stress relative enzymes (PERK, IRE1 ATF6, GRP94, CHOP and caspase-12), (B) autophagy related enzymes (LC3-II/I and Beclin 1), (C) LKB1/CaMKK–AMPK signaling, and (D) apoptosis-related protein (Bcl-2, Bax and caspase-3) levels in the lungs of LPS challenge ALI. Lung tissue extracts were subjected to Western blot analysis using antibodies for PERK, IRE1 ATF6, GRP94, CHOP, caspase-12, LC3-II/I, Beclin 1, LKB1, CaMKK, AMPK, Bcl-2, Bax and caspase-3. Data are represented as means ± S.E.M. (n = 3). ^## p < 0.01 and ^### p < 0.001 versus the control group. * p < 0.05, ** p < 0.01 and *** p < 0.001 versus the LPS group.

Effects of hispolon on (A) ER stress relative enzymes (PERK, IRE1 ATF6, GRP94, CHOP and caspase-12), (B) autophagy related enzymes (LC3-II/I and Beclin 1), (C) LKB1/CaMKK–AMPK signaling, and (D) apoptosis-related protein (Bcl-2, Bax and caspase-3) levels in the lungs of LPS challenge ALI. Lung tissue extracts were subjected to Western blot analysis using antibodies for PERK, IRE1 ATF6, GRP94, CHOP, caspase-12, LC3-II/I, Beclin 1, LKB1, CaMKK, AMPK, Bcl-2, Bax and caspase-3. Data are represented as means ± S.E.M. (n = 3). ^## p < 0.01 and ^### p < 0.001 versus the control group. * p < 0.05, ** p < 0.01 and *** p < 0.001 versus the LPS group.