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Hispolon downregulated ROS (A), a ROS inhibitor (NAC) reduced ROS (B) in the BALF and regulated p-AMPK, HO-1 and nuclear Nrf2 (C) protein expression in the lungs. ROS was detected after LPS challenge by ELISA. Lung tissue extracts were subjected to Western blot analysis using antibodies for p-AMPK, HO-1 and nuclear Nrf2. Data are represented as means ± S.E.M. (n = 3). ## p < 0.01 and ### p < 0.001 versus the control group. ** p < 0.01 and *** p < 0.001 versus the LPS group.
