Figure 1.

Changes produced in B11 tumor cells by the Fhit gene transfection. (A) MHC class I surface expression of B11 and TB11-Fhit tumor cell lines in baseline conditions: H-2 K^d (gray line), H-2 D^d (dotted line), and H-2 L^d (black line). B11 is negative in baseline conditions; TB11-Fhit recovered surface expression of all three H-2 molecules. Data from one experiment are depicted; (B) Transcription levels of Fhit, H-2 class I heavy chain, β₂microglobulin, and several APM components detected by real-time RT-PCR; (C) Transcriptional expression of IFN-γ signal transduction pathway genes in B11 and TB11-Fhit tumor cell lines. Data were normalized using β-actin and GAPDH as housekeeping genes. Only genes with changes in their expression are depicted. Data for B11 are set at 1. Values are depicted as means ± SD of three independent experiments performed in quadruplicate. * p < 0.05. A two-tailed Student’s t-test was used for statistical analysis.