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. 2020 Jun 15;21(12):4246. doi: 10.3390/ijms21124246

Figure 1.

Figure 1

(a) Schematic design of pyrosequencing to detect the E211K somatic mutation of the bovine prion protein gene (PRNP). (b) Gene-specific primers were used to obtain 155 bp amplicons of the bovine PRNP gene containing K211 mutation and E211 wild type. Marker: 100 bp DNA ladder marker; Mut: K211 mutation sample; CTL: E211 normal sample. (c) Electropherograms of Sanger sequencing results. The upper panel indicates Sanger sequencing results of the DNA amplicon with 100% E211 normal sample. The middle panel indicates Sanger sequencing results of mixed DNA amplicons with 50% E211 normal sample and 50% K211 mutation sample. The lower panel indicates Sanger sequencing results of DNA amplicon with 100% K211 mutation sample.