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. 2020 Jun 19;21(12):4356. doi: 10.3390/ijms21124356

Figure 7.

Figure 7

Expression of GLI1 and THY1 in CMSC during osteogenic induction. Markers’ expression analysed by immunofluorescence in (a–c) N-CMSC and (e–g) P-CMSC, cultured with osteogenic medium (OM) for one (1 wk) and three weeks (3 wk), compared to cells cultured in growth medium (GM). Nuclei are stained in blue with DAPI. The intensity of fluorescence was quantified by the ImageJ software for both (d) N-CMSC and (h) P-CMSC. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Pictures are representative of 30 microscopic fields of three different cell culture samples (see Methods for details).