Figure 10.

AXIN2 knockdown results. Expression analysis of: (a) AXIN2; (b) RUNX2; (c) ALP; (d) ON; (e) OPN; and (f) OCN, evaluated by qPCR upon AXIN2 silencing in N-CMSC (si-N-CMSC) cultured with osteogenic medium for one week (OM 1wk). Untreated N-CMSC were used as controls for both si-N-CMSC and P-CMSC. The graphs show the average values derived from three different experiments on three different patient’s derived cells. RQ, Relative Quantity (see Methods for details). * p < 0.05; ** p < 0.01; *** p < 0.001.