Skip to main content
. 2020 Jun 10;12(6):1521. doi: 10.3390/cancers12061521

Figure 3.

Figure 3

Compressive stimulation of ovarian cancer cells causes significant changes in proliferation, cell death, and gene regulation. Cellular proliferation of (A) OVCAR3 and (B) OVSAHO cells under static and cyclic compressive stress stimulation for 24 h (IHC ki67 expression). Cell death of (C) OVCAR3 and (D) OVSAHO cells under static and cyclic compressive stress stimulation for 24 h (IHC cleaved caspase 3 expression). (Significance calculated via t-test; n ≥ 3 experimental replicates, **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.1). (E) Gene expression changes via RT-qPCR for ovarian cancer cells stimulated for 24 h under static or cyclic compressive conditions. A two-fold upregulation is indicated by the dotted line. (F) Representative IHC images of OVCAR3 expression of cleaved caspase 3 and ki67 under compressive stress stimulation for 24 h.