Figure 1.

Sensitivity of GBM cells to Si306 and pro-Si306 and its relation to Src protein expression. (a) Histogram and flow-cytometric profiles representing Src expression in GBM cell lines (U87 and U87-TxR) and primary GBM cells (GBM-4, GBM-5 and GBM-6). Histogram shows mean fluorescence intensity (arbitrary units) obtained by flow-cytometric detection of immunolabeled cells. Values are expressed as mean ± SEM. (b) Sensitivity of GBM cell lines (U87 and U87-TxR) [28] and primary GBM cells (GBM-4, GBM-5 and GBM-6) to Src inhibitors Si306 and pro-Si306 expressed as half maximal inhibitory concentration (IC₅₀) values ± SD (n = 3). The IC₅₀ values were determined using the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.