VASH2 deficiency accelerated neutrophil infiltration in I/R-induced kidney injury. (a) Immunohistochemical images of Ly-6B.2, a marker for neutrophils, in the kidney (original magnification, ×200). (b) The number of Ly-6B.2-positive cells was higher in V2KO-I/R mice than WT-I/R mice; (c) Cxcl2 and (d) Cxcl5 mRNA expression in the kidney. Data were normalized with the expression of 18S rRNA. (e) Immunohistochemical images of CXCL2 (original magnification, ×200). CXCL2-positive tubules are indicated by arrows. (f) The number of CXCL2-positive tubules was higher in V2KO-I/R mice than WT-I/R mice. n = 6 for each group. * p < 0.01 vs. WT-cont, #
p < 0.05 vs. WT-I/R. Each column shows the mean ± SD.