Figure 4.

VASH2 deficiency accelerated I/R-induced peritubular capillary (PTC) loss in the kidney. (a) Immunohistochemical images of the endothelial marker CD34 in the kidney (original magnification, ×200). (b) The decrease in the number of CD34-positive PTCs was greater in V2KO-I/R mice than WT-I/R mice; (c) Vegfa and (d) Icam1 mRNA expression in the kidney. Data were normalized with expression of 18S rRNA. (e,f) Immunoblots to quantify protein levels of VEGF-A and ICAM-1, respectively. VEGF-A bands were observed at 24 kDa. Each lane was loaded with 40 µg of protein. (g,h) Densitometric analysis of the immunoblots. Data were normalized to β-actin. n = 6 for each group. * p < 0.01 vs. WT-cont, ^# p < 0.05 vs. WT-I/R. Each column shows the mean ± SD.