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. 2020 Jun 26;21(12):4545. doi: 10.3390/ijms21124545

Figure 5.

Figure 5

Endogenous VASH2 expression in the kidney in response to I/R injury. (a) Vash2 mRNA levels determined by real-time PCR in the kidneys from WT-cont and WT-I/R mice. n = 6 for each group. Data were normalized with the expression of 18S rRNA. (b) Immunofluorescence staining for β-galactosidase (β-gal) to detect β-gal expression in the kidneys from V2KO-cont (left) and V2KO-I/R (right) mice. For nuclear staining, 4′,6-diamidino-2- phenylindole (DAPI) was used (original magnification, × 200). * p < 0.01 vs. WT-cont. The column shows the mean ± SD.