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. 2020 Jun 2;12(6):1450. doi: 10.3390/cancers12061450

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Nanoparticle tracking analysis (NTA) of EVs derived from plasma of ccRCC patients. The red error bars indicate ± 1 standard error of the mean. (B) Transmission electron microscopy (TEM) of EVs from purified platelet-free plasma (PFP). The TEM image was acquired in the Histology and Electron Microscopy platform from I3S Porto using a Transmission Electron Microscope Jeol JEM 1400. (C) Proportion of fluorescent EVs as observed by flow cytometry from a sample EV isolate derived from a patient, either previously stained with CFSE (left panel) or without previous staining (right panel).